TRITERPENE GLYCOSIDES OF POLYSCIAS SPP. CELL CULTURES
Abstract
Pol 1 was a bidesmoside of oleanolic acid containing three residues of β-D-glucopyranose and one residue of β-D-glucuronepyranose. β-configuration of anomeric centers of monosaccharides was determined in accordance with high values of spin-spin coupling constants (КССВ) J1,2 (6.8-7.8 Hz) and presence in ROESY experiment of typical for β-sugar of correlation peaks H1/H3,H5. Connection places of carbohydrate elements to aglycon as well as consequence of monosaccharides combination with each other were determined by means of ROESY and HMBC experiments. Pol 1 has a structure of 28-О-β-D-glupiranosyl ether 3-О-β-D- glucopyranosyl -(1→4)-O-[β-D- glucopyranosyl -(1→2)]-O-β-D-glucuronopyranoside of oleanolic acid and is identic to polyscioside E, earlier discovered in the roots of Polyscias fruticosa, leaves of P. scutellaria and P. guilfoylei. It was revealed there was polyscioside Е both in the biomass of P. filicifolia («new strain») and in P. fruticosa. At that, the biggest contain of this glycoside (14th day of growth) was found in P. filicifolia («new strain »). According to TLC and HPLC, other glycosides, found in the biomass of the studied cells, were identic to glycosides of Pol 3, Pol 5 and Pol 7 from the leaves of P. filicifolia. Nevertheless, their structure needs to be studied yet. In P. filicifolia («new strain») the main glycoside was Pol 3 (together with polyscioside Е) and in P. fruticosa — Pol 3 and Pol 7.
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